Malathion-induced inhibition of human plasma cholinesterase studied by the fluorescence spectroscopy method
Објеката
- Тип
- Рад у часопису
- Верзија рада
- објављена верзија
- Језик
- енглески
- Креатор
- V. M. Pavelkić, K. S. Krinulović, J. Z. Savić, M. A. Ilić
- Извор
- Russian Journal of Physical Chemistry A
- Издавач
- Pleiades Publishing Ltd
- Датум издавања
- 2008
- Сажетак
-
The in vitro effect of technical grade malathion was assessed via the kinetic parameters of human plasma butyrylcholinesterase (BChE) using N-methylindoxyl acetate as a substrate for BChE. An inhibitor kinetics study demonstrated the existence of a biphasic inhibition curve, indicating high- and low-affinity binding sites of malathion. The IC50 values as calculated from the experimental inhibition curves were 1.33 × 10–9 and 1.48 × 10–5 M for the high- and low-affinity binding sites, respectively; Hill’s analysis gave 1.29 × 10–9 and 1.38 × 10–6 M. The Cornish–Bowden plots and their secondary plots indicated that the nature of inhibition was of mixed type with the predominant competitive character of both affinity binding sites.
- том
- 82
- Број
- 5
- почетак странице
- 870
- крај странице
- 874
- doi
- 10.1134/S0036024408050312
- issn
- 0036-0244
- Subject
- Малатион, инхибиција, хумана плазма холинестераза, метода флуоресцентне спектроскопије
- Malathion, inhibition, human plasma cholinesterase, fluorescence spectroscopy method
- Шира категорија рада
- M20
- Ужа категорија рада
- М23
- Права
- Отворени приступ
- Лиценца
- All rights reserved
- Формат
- Медија
s0036024408050312.pdf
V. M. Pavelkić, K. S. Krinulović, J. Z. Savić, M. A. Ilić. "Malathion-induced inhibition of human plasma cholinesterase studied by the fluorescence spectroscopy method" in Russian Journal of Physical Chemistry A, Pleiades Publishing Ltd (2008). https://doi.org/10.1134/S0036024408050312
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